Anti-CD3 Antibody [CA17.2A12] (FITC) (A279975)

Anti-CD3 Antibody [CA17.2A12] (FITC)

Mouse monoclonal [CA17.2A12] antibody to CD3 (FITC).

This antibody recognises the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin.

Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood. Note: MCA1774F should not be used with MCA1781PE (mouse anti canine B-cells), in dual colour FC, due to non-specific interactions between the two reagents.

Canine

Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-90.

Mouse

Monoclonal

CA17.2A12

Protein G affinity chromatography of ascites.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Dog CD3 antibody, clone CA17.2A12 recognizes the canine CD3 cell surface antigen, expressed by thymocytes and mature T lymphocytes. CD3 is engaged in the surface expression of the T-cell antigen receptor (TCR) and the signal transduction pathway resulting from MHC ligand binding to the TCR. CD3 is made up of a number of invariant subchains of the immunoglobulin superfamily. Mouse anti Dog CD3 clone CA17.2A12 is a valuable flow cytometric and immunohistologic tool for canine lymphoma detection of T-cell origin (Miniscalco et al. 2003).

CD3d, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain, T3D

This product is for research use only. It is not intended for diagnostic or therapeutic use.