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Anti-CD28 (phospho Tyr218) Antibody (A94934)

Western Blot - Anti-CD28 (phospho Tyr218) Antibody (A0845) - Antibodies.com
$185
Product Datasheet

Rabbit polyclonal antibody to CD28 (phospho Tyr218) for WB and ELISA.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-CD28 (phospho Tyr218) Antibody
Description
Rabbit polyclonal antibody to CD28 (phospho Tyr218).
Specificity
This antibody detects endogenous levels of CD28 only when phosphorylated at Tyr218.
Applications
WB, ELISA
Dilutions
WB: 1:500-1:1000, ELISA: 1:40000
Reactivity
Human, Mouse
Immunogen
Synthetic peptide derived from human CD28 around the phosphorylation site of Tyr218 (amino acids 171-220).
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.
Molecular Weight
25kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
T-cell-specific surface glycoprotein CD28, TP44
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (1)

Western Blot - Anti-CD28 (phospho Tyr218) Antibody (A0845) - Antibodies.comEnlarge Image

Western Blot - Anti-CD28 (phospho Tyr218) Antibody (A94934)

Western blot analysis of lysates from HepG2 cells treated with nocodazole 1µg/ml 16h using Anti-CD28 (phospho Tyr218) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.
Publishing research using Anti-CD28 (phospho Tyr218) Antibody (A94934)? Please let us know so that we can list the citation on this page.

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