Anti-CD28 Antibody [E18] (PE) (A280303)

$515

Mouse monoclonal [E18] antibody to CD28 (PE) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-CD28 Antibody [E18] (PE)
Description
Mouse monoclonal [E18] antibody to CD28 (PE).
Specificity
This antibody recognises mouse CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use10ul of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Mouse
Immunogen
Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.
Host
Mouse
Clonality
Monoclonal
Clone ID
E18
Isotype
IgG2b
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Mouse CD28 antibody, clone E18 recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Synonyms
T-cell-specific surface glycoprotein CD28, TP44
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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