Anti-CD28 Antibody [E18] (FITC) (A280304)

Anti-CD28 Antibody [E18] (FITC)

Mouse monoclonal [E18] antibody to CD28 (FITC).

This antibody recognises mouse CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.

Flow Cytometry, Functional Assays

Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Mouse

Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.

Mouse

Monoclonal

E18

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Mouse CD28 antibody, clone E18 recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.

T-cell-specific surface glycoprotein CD28, TP44

This product is for research use only. It is not intended for diagnostic or therapeutic use.