Mouse monoclonal [CD28.2] antibody to CD28 (PE-Cyanine 7).
Specificity
This antibody recognizes an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of MW 44 kDa) which is a critical costimulatory receptor of T cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA.
Host
Mouse
Clonality
Monoclonal
Clone ID
CD28.2
Isotype
IgG1
Light Chains
kappa
Conjugate
PE-Cyanine 7
Excitation: 565nm, Emission: 778nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
44 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!