Anti-CD28 Antibody [CC219] - BSA and Azide free (A280020)

Anti-CD28 Antibody [CC219] - BSA and Azide free

Mouse monoclonal [CC219] antibody to CD28.

This antibody recognises the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells. Mouse anti Bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3. For functional assays it is recommended to use the low endotoxin and preservative free format of this antibody, MCA5779EL. Mouse anti Bovine CD28, clone CC219 has previously been published under the alternative clone designation F848EC4.

Flow Cytometry, Functional Assays

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl

Bovine

Plasmid DNA encoding the bovine CD28 gene.

Mouse

Monoclonal

CC219

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Bovine CD28, clone CC219, recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells.Mouse anti Bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3. For functional assays it is recommended to use the low endotoxin and preservative free format of this antibody, MCA5779EL.Mouse anti Bovine CD28, clone CC219 has previously been published under the alternative clone designation F848EC4 (Hogg et al. 2011).

T-cell-specific surface glycoprotein CD28, TP44