Anti-CD28 Antibody [CC219] (A280021)

$355

Mouse monoclonal [CC219] antibody to CD28 for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-CD28 Antibody [CC219]
Description
Mouse monoclonal [CC219] antibody to CD28.
Specificity
This antibody recognises the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells. Mouse anti Bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3. For functional assays it is recommended to use the low endotoxin and preservative free format of this antibody, MCA5779EL. Mouse anti Bovine CD28, clone CC219 has previously been published under the alternative clone designation F848EC4.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Bovine
Immunogen
Plasmid DNA encoding the bovine CD28 gene.
Host
Mouse
Clonality
Monoclonal
Clone ID
CC219
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Bovine CD28, clone CC219, recognizes the bovine homologue of human CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). CD28 is involved in increased production of IL-4 and IL-10 in T cells.Mouse anti Bovine CD28, clone CC219 is able to stimulate bovine T cells in combination with anti-bovine CD3. For functional assays it is recommended to use the low endotoxin and preservative free format of this antibody, MCA5779EL.Mouse anti Bovine CD28, clone CC219 has previously been published under the alternative clone designation F848EC4 (Hogg et al. 2011).
Synonyms
T-cell-specific surface glycoprotein CD28, TP44
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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