This antibody recognises the chicken homologue of mammalian CD28, demonstrating 50% sequence identity and conservation of key functional sequence. In chickens CD28 is expressed by all thymocytes and T cells excluding most of those that are gamma/delta TCR positive. The co-stimulatory role of CD28 is important to T cell function in chickens. Mouse anti chicken CD28, clone AV7 has been demonstrated to recognise Turkey CD28.
Applications
Flow Cytometry, IP
Dilutions
Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Chicken, Turkey
Immunogen
Chicken Leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
AV7
Isotype
IgG1
Conjugate
Unconjugated
Purification
Ion exchange chromatography of tissue culture supernatant.
Concentration
500 µg/ml
Product Form
Liquid
Formulation
Supplied in Borate Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Chicken CD28, clone AV7 recognizes the chicken homologue of mammalian CD28, demonstrating 50% sequence identity and conservation of key functional sequence. In chickens CD28 is expressed by all thymocytes and T cells excluding most of those that are gamma/delta TCR positive (Koskela et al. 1998). The co-stimulatory role of CD28 is important to T cell function in chickens (Arstila et al. 1994).Mouse anti chicken CD28, clone AV7 has been demonstrated to recognise Turkey CD28 (Powell et al. 2009).