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Anti-CD28 Antibody [ARC5082-01] (A309309)

Western Blot - Anti-CD28 Antibody [ARC5082-01] (A309309) - Antibodies.com Flow Cytometry - Anti-CD28 Antibody [ARC5082-01] (A309309) - Antibodies.com
$540
Product Datasheet

Rabbit monoclonal [ARC5082-01] antibody to CD28 for WB and Flow Cytometry.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-CD28 Antibody [ARC5082-01]
Description
Rabbit monoclonal [ARC5082-01] antibody to CD28.
Applications
WB, Flow Cytometry
Dilutions
WB: 1:500-1:1,000, Flow Cytometry: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant protein of human CD28.
Host
Rabbit
Clonality
Monoclonal
Clone ID
ARC5082-01
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
45 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol, 0.05% BSA, and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
T-cell-specific surface glycoprotein CD28, TP44
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (2)

Western Blot - Anti-CD28 Antibody [ARC5082-01] (A309309) - Antibodies.comEnlarge Image

Western Blot - Anti-CD28 Antibody [ARC5082-01] (A309309)

Western blot analysis of extracts of various cell lines, using Anti-CD28 Antibody [ARC5082-01] (A309309) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 90s.
Flow Cytometry - Anti-CD28 Antibody [ARC5082-01] (A309309) - Antibodies.comEnlarge Image

Flow Cytometry - Anti-CD28 Antibody [ARC5082-01] (A309309)

Flow cytometric analysis of Anti-CD28 Antibody [ARC5082-01] (A309309) (10 µg/ml) in Jurkat cells (orange) compared to Alexa Fluor 647 conjugated goat anti-rabbit polyclonal antibody (1:600 dilution) Isotype control (blue) and non-staining control (red).
Publishing research using Anti-CD28 Antibody [ARC5082-01] (A309309)? Please let us know so that we can list the citation on this page.

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