CD28 expression in Human Tonsil lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-CD28 Antibody (A83546) at 0.3µg/ml and detected by chemiluminescence.
CD28 expression in Human Lymph Node analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by microwaving in citrate buffer, pH 6. Staining was performed with Anti-CD28 Antibody (A83546) at 2µg/ml and revealed with horseradish peroxidase (HRP).
Negative control for CD28 expression in Human Lymph Node analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.
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