Mouse monoclonal [LT27] antibody to CD27 (APC-Cyanine 7).
Specificity
This antibody reacts with an extracellular epitope of CD27, a 50-55 kDa type I transmembrane glycoprotein, of the TNF-receptor superfamily, expressed on medullary thymocytes, peripheral T lymphocytes, some B lymphocytes, and NK cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human peripheral blood lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
LT27
Isotype
IgG2a
Conjugate
APC-Cyanine 7
Excitation: 650nm, Emission: 774nm
Purification
This antibody is conjugated with tandem dye APC-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
50-55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD27 Antibody [LT27] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
Separation of human CD27 positive lymphocytes (red-filled) from monocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD27 Antibody [LT27] (APC-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
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