Anti-CD27 Antibody [B30C7] (A279759)

Anti-CD27 Antibody [B30C7]

Mouse monoclonal [B30C7] antibody to CD27.

This antibody recognises the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family. In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however, using the B30C7 clone, expression of CD27 on porcine B-cells appears undetectable. Porcine CD27 is expressed by all naïve CD8a-ve T-helper cells and a sub-population of CD8a+ve cells. Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and clone B30C7 may be used to differentiate between subsets of pig NK cells.

Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Porcine

Porcine Peripheral Blood Monocytes.

Mouse

Monoclonal

B30C7

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD27 antibody, clone B30C7 recognizes the porcine homologue of human CD27, previously known as Swine Workshop Cluster 2 (SWC2), a T-cell co-stimulatory molecule belonging to the TNF receptor family. In humans the CD27 antigen is expressed by discrete populations of T- and B-cells where it functions in a co-stimulatory role to induce proliferation of T-cells and B-cells, however, using the B30C7 clone, expression of CD27 on porcine B-cells appears undetectable (Reutner et al. 2012).Porcine CD27 is expressed by all naïve CD8a-ve T-helper cells and a sub-population of CD8a+ve cells (Reutner et al. 2012).Monoclonal antibodies to CD27 have previously been used to differentiate between subsets of NK cells and clone B30C7 may be used to differentiate between subsets of pig NK cells (Mair et al. 2013).

CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, TNFRSF7, Tumor necrosis factor receptor superfamily member 7