This antibody recognises CD23 also known as Fc?RII (Fc epsilon Receptor II). CD23 is a ~45 kDa cell surface glycoprotein that acts as a low affinity receptor for IgE.CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human
Immunogen
Immunoprecipitated CD23 antigen from purified plasma membranes from EBV-transformed human lymphoblastoid B cell lines.
Host
Mouse
Clonality
Monoclonal
Clone ID
D3.6
Isotype
IgG2a
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD23 antibody, clone D3.6 recognizes CD23 also known as Fc?RII (Fc epsilon Receptor II). CD23 is a ~45 kDa cell surface glycoprotein that acts as a low affinity receptor for IgE.CD23 is expressed by B cells in the follicular mantle but not by proliferating germinal centre cells. CD23 is also expressed by eosinophils.
Synonyms
BLAST-2, C-type lectin domain family 4 member J, CD23A, CLEC4J, Fc-epsilon-RII, FCE2, FCER2, IGEBF, Immunoglobulin E-binding factor, Low affinity immunoglobulin epsilon Fc receptor, Lymphocyte IgE receptor