Anti-CD227 (phospho Tyr1229) Antibody (A94238)

Name: Anti-CD227 (phospho Tyr1229) Antibody
Brand: Antibodies.com
SKU: A94238
Price: 185.0 USD
Availability: InStock

Enlarge Image

$185

Rabbit polyclonal antibody to CD227 (phospho Tyr1229) for WB, IHC, IF and ELISA.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Specifications

Name

Anti-CD227 (phospho Tyr1229) Antibody

Description

Rabbit polyclonal antibody to CD227 (phospho Tyr1229).

Specificity

This antibody detects endogenous levels of CD227/MUC1 only when phosphorylated at Tyr1229.

Applications

WB, IHC, IF, ELISA

Dilutions

WB: 1:500-1:1000, ELISA: 1:5000

Reactivity

Human

Immunogen

Synthetic peptide derived from human CD227/MUC1 around the phosphorylation site of Tyr1229 (amino acids 1201-1250).

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from rabbit serum by antigen affinity chromatography using the immunizing phospho peptide.

Molecular Weight

122kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline (without Mg2+ and Ca2+), pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Breast carcinoma-associated antigen DF3, CA 15-3, Cancer antigen 15-3, Carcinoma-associated mucin, CD227, Episialin, H23AG, MUC-1, Mucin-1, PUM

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

Enlarge Image

Immunofluorescence - Anti-CD227 (phospho Tyr1229) Antibody (A94238)

Immunofluorescence analysis of HepG2 cells using Anti-CD227 (phospho Tyr1229) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

Enlarge Image

Immunohistochemistry - Anti-CD227 (phospho Tyr1229) Antibody (A94238)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Anti-CD227 (phospho Tyr1229) Antibody. The right hand panel represents a negative control, where the antibody was pre-incubated with the immunising peptide.

Enlarge Image

Western Blot - Anti-CD227 (phospho Tyr1229) Antibody (A94238)

Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 30' using Anti-CD227 (phospho Tyr1229) Antibody. The right hand lane represents a negative control, where the antibody is blocked by the immunising peptide.

Publishing research using Anti-CD227 (phospho Tyr1229) Antibody (A94238)? Please let us know so that we can list the citation on this page.