This antibody recognises the human CD22 cell surface antigen, a ~130/140 kDa glycoprotein expressed by cells of B lymphocyte lineage.CD22 is initially expressed cytoplasmically and subsequently on the cell surface during B cell maturation. Expression is lost upon terminal differentiation to plasma cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 6 cells in 100µl.
Reactivity
Human
Immunogen
Burkitt's lymphoma cell line Raji.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-01
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD22 antibody, clone MEM-01 recognizes the human CD22 cell surface antigen, a ~130/140 kDa glycoprotein expressed by cells of B lymphocyte lineage.CD22 is initially expressed cytoplasmically and subsequently on the cell surface during B cell maturation. Expression is lost upon terminal differentiation to plasma cells.