This antibody recognises the human CD22 cell surface antigen, a ~130/140 kDa glycoprotein expressed by cells of B lymphocyte lineage.CD22 is initially expressed cytoplasmically and subsequently on the cell surface during B cell maturation. Expression is lost upon terminal differentiation to plasma cells.
Applications
WB, IP, Flow Cytometry
Dilutions
Flow Cytometry: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human
Immunogen
Burkitt's lymphoma cell line Raji.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-01
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Molecular Weight
Approximately 140kD in cell lysates under non reducing conditions.
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD22 antibody, clone MEM-01 recognizes the human CD22 cell surface antigen, a ~130/140 kDa glycoprotein expressed by cells of B lymphocyte lineage.CD22 is initially expressed cytoplasmically and subsequently on the cell surface during B cell maturation. Expression is lost upon terminal differentiation to plasma cells.