Recombinant rabbit monoclonal [DM13] antibody to CD22.
Applications
ELISA, Flow Cytometry
Dilutions
Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM13
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Expi 293 cell line was transfected with irrelevant protein (A) and human CD22 (B) were surface stained with Anti-CD22 Antibody [DM13] - BSA and Azide free (A318673) at 1µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Flow cytometry data of serially titrated Anti-CD22 Antibody [DM13] - BSA and Azide free (A318673) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit Anti-CD22 Monoclonal Antibody clones by titration of different concentrations onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Anti-CD22 Antibody [DM13] - BSA and Azide free (A318673)
Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 Monoclonal Antibody clones. A) Heavy chain and B) Light chain.
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