Western Blot - Anti-CD204 Antibody [ARC0750] (A307271)
Western blot analysis of extracts of various cell lines, using Anti-CD204 Antibody [ARC0750] (A307271) at 1:1,000 dilution. C6 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded rat brain using Anti-CD204 Antibody [ARC0750] (A307271) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse testis using Anti-CD204 Antibody [ARC0750] (A307271) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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