Anti-CD204 Antibody [2F8] - BSA and Azide free (A279223)

Anti-CD204 Antibody [2F8] - BSA and Azide free

Rat monoclonal [2F8] antibody to CD204.

This antibody recognises the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. recognises an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain.

Flow Cytometry, WB, ELISA, IHC-Fr, IP, Functional Assays

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Mouse, Pig, Channel catfish

Raw 264 cell line.

Rat

Monoclonal

2F8

IgG2b

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Rat anti Mouse CD204 antibody, clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993). Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).

Macrophage acetylated LDL receptor I and II, Macrophage scavenger receptor types I and II, SCARA1, Scavenger receptor class A member 1