Mouse monoclonal [OX-102] antibody to CD200R (PE).
Specificity
This antibody recognises the rat OX2 (CD200) receptor 1. This antigen is a heavily glycosylated ~60-100 kDa cell surface molecule expressed by cells of the myeloid lineage but not by T or B lymphocytes. Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 has been shown to block the interaction of OX2 receptor 1 with CD200.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Rat
Immunogen
Membrane fraction of thioglycollate-elicited rat peripheral cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-102
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 recognizes the rat OX2 (CD200) receptor 1. This antigen is a heavily glycosylated ~60-100 kDa cell surface molecule expressed by cells of the myeloid lineage but not by T or B lymphocytes.Mouse anti Rat CD200 Receptor 1 antibody, clone OX-102 has been shown to block the interaction of OX2 receptor 1 with CD200 (Bushell et al. 2008).
