Anti-CD20 Antibody [SPM494] (A254286)

Anti-CD20 Antibody [SPM494]

Mouse monoclonal [SPM494] antibody to CD20.

This antibody recognizes a protein of 30-33kDa, which is identified as CD20. Its epitope is located in the cytoplasmic domain of CD20 and was, therefore, ascribed as CD20cy in the 5th Workshop. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Flow Cytometry, IF, IHC-P

Flow Cytometry: 2-5µg/million cells, IF: 1:100-1:200, IHC-P: 2-4 µg/ml

Human, Baboon, Monkey

This antibody does not cross react with Bovine, Canine, Porcine, or Rat.

Human tonsil B cells.

Mouse

Monoclonal

SPM494

kappa

33-37 kDa

Tissue culture supernatant.

Liquid

Supplied as an aliquot of tissue culture supernatant with 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, MS4A1