This antibody reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.
Applications
Flow Cytometry, IP
Dilutions
Flow Cytometry: 1-5 µg/ml.
Reactivity
Bovine, Human
Immunogen
Raji human Burkitt's lymphoma cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-97
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
33-37 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Immunoprecipitation of human CD20 from the whole cell lysate of RAJI human Burkitt lymphoma cell line. Western blot was immunostained with Anti-CD20 Antibody (rabbit polyclonal). Lane 1: Original lysate of RAJI cells. Lane 2: Immunoprecipitate by Isotypic mouse IgG1 control (PPV-06). Lane 3: Original lysate of RAJI cells. Lane 4: Immunoprecipitate by Anti-CD20 Antibody (A85848).
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