Anti-CD20 Antibody [FMC7] (FITC) (A280482)

Anti-CD20 Antibody [FMC7] (FITC)

Mouse monoclonal [FMC7] antibody to CD20 (FITC).

This antibody recognises a glycoprotein antigen of ~105 kDa expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes. recognises a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20. Identity of CD20 as the antigen recognized by Mouse anti Human B cells antibody, clone FMC7 was further confirmed by strong recognition of recombinant CD20 expressed in hematopoietic and non-haematopoietic cell lines and abolition of binding in CD20 extracellular domain mutations. The recognized epitope has also been shown to be cholesterol dependent.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood.

Human

HRIK cells - human B-Lymphoblastoid line.

Mouse

Monoclonal

FMC7

Gel filtration of ascites.

Lot Specific

Liquid

Supplied in TRIS buffered glycine with 0.1% Sodium Azide and 0.2% BSA.

Shipped at ambient temperature. Store at 4°C. This product is photosensitive and should be protected from light.

Mouse anti Human B cells antibody, clone FMC7 recognizes a glycoprotein antigen of ~105 kDa expressed by B lymphocytes. The FMC7 antigen is expressed by peripheral B lymphocytes. Mouse anti Human B cells antibody, clone FMC7 has been used extensively to differentiate various types of B cell malignancy. B-CLL is generally considered to be negative for FMC7 expression, but strong staining is seen in many other types of B cell lymphoma, including prolymphocytic leukemia and hairy cell leukemia.The expression pattern of the FMC7 antigen closely corresponds to that seen with CD22. Mouse anti Human B cells antibody, clone FMC7 recognizes a conformational epitope on the CD20 molecule, most likely a multimeric complex of CD20 (Serke et al. 2001). Identity of CD20 as the antigen recognized by Mouse anti Human B cells antibody, clone FMC7 was further confirmed by strong recognition of recombinant CD20 expressed in hematopoietic and non-haematopoietic cell lines and abolition of binding in CD20 extracellular domain mutations. The recognized epitope has also been shown to be cholesterol dependent (Polyak et al. 2003).

This product is for research use only. It is not intended for diagnostic or therapeutic use.