Anti-CD20 Antibody [B9E9] (A250593)

Anti-CD20 Antibody [B9E9]

Mouse monoclonal [B9E9] antibody to CD20.

This antibody recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 This antibody recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.

Functional Studies, Flow Cytometry, IF

Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Human

Lymphoblastoid cell line Daudi.

Mouse

Monoclonal

B9E9

IgG2a

Protein A/G chromatography.

200 µg/ml

33-37 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD20 Antibody [B9E9] - BSA and Azide free (A253773).