This antibody recognises the rat beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. CD18 is expressed by all leucocytes. Mouse anti Rat CD18 antibody has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
FTL-43.
Host
Mouse
Clonality
Monoclonal
Clone ID
WT.3
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Rat CD18 antibody, clone WT.3 reacts with the rat beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. CD18 is expressed by all leucocytes. Mouse anti Rat CD18 antibody has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1.