Mouse monoclonal [MEM-48] antibody to CD18 (FITC).
Specificity
This antibody recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen. CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
Reactivity
Human
Cross Reactivity
This antibody does not cross react with Dog.
Immunogen
Leukocytes from a patient suffering from a LGL Type leukemia.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-48
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
90-95 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!