CD18 expression in K562 (A) and U937 (B) lysates analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary antibody incubation was performed with Anti-CD18 Antibody (A83631) at 0.3µg/ml and detected by chemiluminescence.
CD18 expression in Human Spleen analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by steaming in citrate buffer, pH 6. Staining was performed with Anti-CD18 Antibody (A83631) at 5µg/ml and revealed with alkaline phosphatase (AP).
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