Mouse monoclonal [MEM-166] antibody to CD177 (APC).
Specificity
This antibody recognises human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 have been implicated in the pathology of neonatal alloimmune neutropenia.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human, Rhesus Monkey
Immunogen
Human granulocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-166
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml after reconstituiton.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD177 antibody, clone MEM-166 recognizes human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 have been implicated in the pathology of neonatal alloimmune neutropenia (Lalezari et al. 1971).
Synonyms
CD177 antigen, HNA-2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, NB1 GP, Polycythemia rubra vera protein 1, PRV-1, PRV1