This antibody recognises human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 have been implicated in the pathology of neonatal alloimmune neutropenia.
Applications
WB, IP, Flow Cytometry
Dilutions
Flow Cytometry: 1:50 - 1:100, WB: Non-reducing conditions, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human, Rhesus Monkey
Immunogen
Human granulocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-166
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Human CD177 antibody, clone MEM-166 recognizes human CD177 (neutrophil glycoprotein NB1). The neutrophil NB1 antigen is expressed by 97% of the caucasian population. Antibodies against NB1 have been implicated in the pathology of neonatal alloimmune neutropenia (Lalezari et al. 1971).
Synonyms
CD177 antigen, HNA-2a, Human neutrophil alloantigen 2a, NB1, NB1 glycoprotein, NB1 GP, Polycythemia rubra vera protein 1, PRV-1, PRV1