Anti-CD14 Antibody [MEM-18] - Low endotoxin, Azide free (A85711)

Anti-CD14 Antibody [MEM-18] - Low endotoxin, Azide free

Mouse monoclonal [MEM-18] antibody to CD14.

This antibody reacts with CD14, a 53-55 kDa GPI-linked extracellular membrane glycoprotein expressed on monocytes, macrophages, and weakly on granulocytes. It is also expressed by most tissue macrophages. In human, the epitope recognized by this antibody is located between amino acids 57-64.

Flow Cytometry, IP, WB, ELISA, Functional Studies

ELISA: This antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 (cat. no. 11-304-C100), FUNC: This antibody completely blocks binding of fluorescein (FITC) labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain, Flow Cytometry: 4 µg/ml, WB: Non-reducing conditions.

Human, Non-Human Primates

A crude mixture of human urinary proteins, precipitated by ammonium sulphate, from the urine of a patient suffering from proteinuria.

Mouse

Monoclonal

MEM-18

IgG1

Protein A chromatography.

1 mg/ml

53-55 kDa

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, without Sodium Azide (0.2 µm filter sterilized). Endotoxin level is less than 0.01 EU/µg of the protein.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein