Anti-CD14 Antibody [MEM-18] (A281114)

Anti-CD14 Antibody [MEM-18]

Mouse monoclonal [MEM-18] antibody to CD14.

This antibody recognises human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognises the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti CD14 antibody, clone UCHM1 as a detection reagentMouse anti Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes and has been used successfully for the detection of soluble CD14 in saliva samples.

WB, IP, Flow Cytometry, ELISA

Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Human

Crude protein prepared by ammonium sulfate precipitation of urine from a proteinuria patient.

Mouse

Monoclonal

MEM-18

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with <0.1% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD14 antibody, clone MEM-18 recognizes human CD14, also known as Myeloid cell-specific leucine-rich glycoprotein. CD14 is a 375 amino acid ~55 kDa GPI-anchored cell membrane protein found predominantly on monocytes and macrophages, it is less strongly expressed on granulocytes, and is absent from stem cells and myeloid cells of very early differentiation states. In immunohistology CD14 present on Langerhans cells, follicular dendritic cells, histocytes and high endothelial venules. In ELISA clone MEM-18 recognizes the soluble form CD14 and has been used successfully in the develpopment of a sensitive ELISA as a capture reagent in conjunction with biotinylated Mouse anti CD14 antibody, clone UCHM1 as a detection reagentMouse anti Human CD14 antibody, clone MEM-18 is reported to block the binding of bacterial lipopolysaccharide (LPS) to monocytes (Prager et al. 2001) and has been used successfully for the detection of soluble CD14 in saliva samples (Bergandi et al. 2007).

Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein