Anti-CD14 Antibody [CC-G33] (FITC) (A280069)

Anti-CD14 Antibody [CC-G33] (FITC)

Mouse monoclonal [CC-G33] antibody to CD14 (FITC).

This antibody recognises bovine CD14.CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules. Mouse anti Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells , ovine CD14 and Water buffalo (Bubalus bubalis) CD14,.

Use 10µl of the suggested working dilution to label 1x10⁶ cells in 100µl.

Bovine, Sheep, Human, Water Buffalo

Partially purified polypeptides isolated from bovine leucocyte cell surface membrane.

Mouse

Monoclonal

CC-G33

IgG1

Protein A affinity chromatography of tissue culture supernatant.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Bovine CD14, clone CC-G33 recognizes bovine CD14.CD14 is a GPI-anchored membrane glycoprotein and monocyte/macrophage differentiation antigen, belonging to the lipopolysaccharide receptor family, also expressed weakly on microglia and Langerhans cells. CD14 acts as a receptor for the potent bacterial endotoxin, lipopolysaccharide (LPS), facilitated by LPS-binding protein (LBP). The binding of LPS to CD14 results in cell activation and the release of cytokines and the inflammatory response, and has been shown to upregulate the cell surface expression of adhesion molecules.Mouse anti Bovine CD14 clone CC-G33 cross-reacts with human CD14 expressed on transfected COS-7 cells (Berthon & Hopkins 1996), ovine CD14 (Sopp et al. 1996) and Water buffalo (Bubalus bubalis) CD14, (Mirielli et al. 2013).

Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein

This product is for research use only. It is not intended for diagnostic or therapeutic use.