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Anti-CD14 Antibody (A9747)

Western Blot - Anti-CD14 Antibody (A9747) - Antibodies.com Immunofluorescence - Anti-CD14 Antibody (A9747) - Antibodies.com Immunofluorescence - Anti-CD14 Antibody (A9747) - Antibodies.com
$335
Product Datasheet

Rabbit polyclonal antibody to CD14 for WB and ICC/IF.

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Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-8 business days.

  • Specifications

Name
Anti-CD14 Antibody
Description
Rabbit polyclonal antibody to CD14.
Applications
WB, ICC/IF
Dilutions
WB: 1:500-1:1,000, ICC/IF: 1:50-1:200
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CD14 (NP_001167576.1).
Sequence
TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNP
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Conjugate

Unconjugated

Purification
Affinity purification.
Molecular Weight
60 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Synonyms
Monocyte differentiation antigen CD14, My23 antigen, Myeloid cell-specific leucine-rich glycoprotein
Isotype Controls
Suitable Secondaries
See all Anti-Rabbit IgG Secondaries →
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.

  • Scientific Validation DataValidation Data (3)

Western Blot - Anti-CD14 Antibody (A9747) - Antibodies.comEnlarge Image

Western Blot - Anti-CD14 Antibody (A9747)

Western blot analysis of extracts of RAW264. 7 cells, using Anti-CD14 Antibody (A9747) at 1:600 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.
Immunofluorescence - Anti-CD14 Antibody (A9747) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-CD14 Antibody (A9747)

Immunofluorescence analysis of RAW264. 7 cells using Anti-CD14 Antibody (A9747) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence - Anti-CD14 Antibody (A9747) - Antibodies.comEnlarge Image

Immunofluorescence - Anti-CD14 Antibody (A9747)

Immunofluorescence analysis of THP-1 cells using Anti-CD14 Antibody (A9747) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Publishing research using Anti-CD14 Antibody (A9747)? Please let us know so that we can list the citation on this page.

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