This antibody recognises human CD137, an 83kD type I integral membrane protein, otherwise known as 4-1BB. The CD137 molecule is primarily expressed on activated T-cells. It is a costimulatory member of the tumour necrosis receptor family and regulates T-cell activation and proliferation.Rat anti Human CD137 antibody, clone JG1.6A induces ?-interferon activity in isolated T-cells as measured by ELISA.
Applications
Flow Cytometry, ELISA
Dilutions
Use 10µl of the suggested working dilution to label 1x106 cells in 100µl.
Reactivity
Human
Immunogen
Recombinant human CD137-Fc.
Host
Rat
Clonality
Monoclonal
Clone ID
JG1.6A
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in TRIS Buffered Saline with <0.1% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Human CD137 antibody, clone JG1.6A recognizes human CD137, an 83kD type I integral membrane protein, otherwise known as 4-1BB. The CD137 molecule is primarily expressed on activated T-cells. It is a costimulatory member of the tumour necrosis receptor family and regulates T-cell activation and proliferation.Rat anti Human CD137 antibody, clone JG1.6A induces ?-interferon activity in isolated T-cells as measured by ELISA (Rajendran et al. 2019).