Recombinant rabbit monoclonal [DM66] antibody to CD137.
Applications
ELISA, Flow Cytometry
Dilutions
ELISA: 1:5,000-10,000, Flow Cytometry: 1:100
Reactivity
Human
Host
Rabbit
Clonality
Monoclonal
Clone ID
DM66
Isotype
IgG
Conjugate
Unconjugated
Purification
Affinity chromatography.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with distilled sterile water.
Formulation
Lyophilized from sterile Phosphate Buffered Saline, pH 7.4. Normally 5%–8% Trehalose is added as a protectant before lyophilization.
Storage
Shipped at 4°C. Lyophilized: Store at -20°C to -80°C. Reconstituted: Aliquot and store at -80°C. Product is stable for one year. Avoid freeze/thaw cycles.
General Notes
Prior to reconstitution, centrifuge the vial at 5,000g for 3-5 minutes at room temperature. Reconstitute with appropriate volume of distilled sterile water to bring product to 1mg/ml concentration. After addition of distilled sterile water, mix by gentle tapping. Note: It is not recommended to vortex or vigorously pipette the sample.
Anti-CD137 Antibody [DM66] - BSA and Azide free (A317400)
ELISA plate pre-coated by 2 µg/ml (100 µl/well) Recombinant Human CD137 Protein (Fc Chimera 6xHis Tag) (A318397) can bind Anti-CD137 Antibody [DM66] - Azide free (A317400) in a linear range of 1-100 ng/ml.
Expi 293 cell line transfected with irrelevant protein (A) and human 4-1BB (B) were surface stained with Anti-CD137 Antibody [DM66] - Azide free (A317400) at 1 µg/ml followed by Anti-Rabbit IgG Antibody (Alexa 488).
Flow cytometry data of serially titrated Anti-CD137 Antibody [DM66] - Azide free (A317400) on Expi 293 cell line transfected with human 4-1BB. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different rabbit monoclonal Anti-4-1BB Antibodies by titration of different concentrations onto Expi 293 cell line transfected with human 4-1BB. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
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