This antibody recognizes an extracellular conformational epitope on CD137, an approximately 40 kDa type I transmembrane protein of the TNFR family expressed mainly on activated T cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Recombinant human CD137 ectodomain.
Host
Mouse
Clonality
Monoclonal
Clone ID
4B4-1
Isotype
IgG1
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
40 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human PHA stimulated peripheral blood mononuclear cell suspension stained with Anti-CD137 Antibody [4B4-1] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
Flow cytometric analysis of human peripheral blood mononuclear cells stained using Anti-CD137 Antibody [4B4-1] (APC) (10µl reagent per milion cells in 100µl of cell suspension) and Anti-CD25 Antibody [MEM-181] (PE) (20µl reagent per milion cells in 100µl of cell suspension).
Separation of cells stained with Anti-CD137 Antibody [4B4-1] (APC) (10µl reagent per milion cells in 100µl of cell suspension, red-filled) from cells stained with Mouse IgG1 [MOPC-21] (APC) (Isotype Control) (concentration in sample is 1µg/ml, same as Anti-CD137 Antibody [4B4-1] (APC) concentration, black-dashed) in flow cytometry analysis of human PHA stimulated peripheral blood mononuclear cell suspension.
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