Rat monoclonal [OX-86] antibody to CD134 / OX40L Receptor (PE).
Specificity
This antibody recognises mouse OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).
Reactivity
Mouse
Immunogen
CHO cells transfected with mouse OX40.
Host
Rat
Clonality
Monoclonal
Clone ID
OX-86
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD134 antibody, clone OX-86 recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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