Rat monoclonal [OX-86] antibody to CD134 / OX40L Receptor (FITC).
Specificity
This antibody recognises mouse OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10, Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/BUF041B).
Reactivity
Mouse
Immunogen
CHO cells transfected with mouse OX40.
Host
Rat
Clonality
Monoclonal
Clone ID
OX-86
Isotype
IgG1
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Rat anti Mouse CD134 antibody, clone OX-86 recognizes murine OX40 (CD134), a cell surface antigen expressed only by activated T lymphocytes.Rat anti Mouse CD134 antibody, clone OX-86 is routinely tested on concanavalin A activated mouse splenocytes.
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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