Mouse monoclonal [W6B3C1] antibody to CD133 (APC).
Specificity
This antibody recognizes the extracellular glycosylated epitope 1 on human CD133, a 120 kDa glycoprotein of prominin family, expressed (for example) on progenitor cells. This antibody is important for identification of stem cells and tumor cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
WERI-RB-1 retinoblastoma cell line.
Host
Mouse
Clonality
Monoclonal
Clone ID
W6B3C1
Isotype
IgG1
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
120 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
Antigen AC133, PROM1, Prominin-1, Prominin-like protein 1, PROML1
Flow cytometric analysis of human bone marrow cells stained using Anti-CD133 Antibody [W6B3C1] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
Flow cytometric analysis of human bone marrow cells stained using Anti-CD133 Antibody [W6B3C1] (APC) (10µl reagent per milion cells in 100µl of cell suspension) and Anti-CD34 Antibody [581] (PE) (20µl reagent per milion cells in 100µl of cell suspension).
Separation of human CD133 positive CD34 positive stem cells (red-filled) from CD133 negative CD34 negative cells (black-dashed) in flow cytometry analysis of human bone marrow cells using Anti-CD133 Antibody [W6B3C1] (APC) (10µl reagent per milion cells in 100µl of cell suspension).
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