Anti-CD133 Antibody [PROM/1510] (A314585)

Anti-CD133 Antibody [PROM/1510]

Mouse monoclonal [PROM/1510] antibody to CD133.

CD133, also known as PROML1 or Prominin, is a stem cell antigen that may be useful for the selection and expansion of hematopoietic cells and may be used as a positive marker for the characterization of trophoblast cell lines. The human CD133 gene maps to chromosome 4p15.32 and encodes an 865 amino acid protein. The CD133 gene codes for a pentaspan transmembrane glycoprotein that is expressed on primitive hematopoietic stem, progenitor, retinoblastoma, hemangioblasts and neural stem cells and developing epithelium. The 5-TM structure includes an extracellular N-terminus, two short intra- cellular loops, two large extracellular loops and an intracellular C-terminus. CD133 is a candidate gene for retinal proteins that are targeted to plasma membrane protrusions. These retinal proteins, including CD133, may influence the shedding of photoreceptive membranes from the terminal end of the outer segments of vertebrate photoreceptors, where they are phagocytosed by the retinal pigment epithelium, and represent candidates for inherited retinal degenerations.

Human tonsil.

IHC: 1-2 µg/ml

Human

Recombinant chimeric PROM1 protein (around Amino Acids 180-380 and aa612-765). This Mab recognizes both glycosylated and non-glycosylated extracellular domains of CD133.

Mouse

Monoclonal

IgG2

kappa

Protein A/G chromatography.

200 µg/ml

97 kDa. Depending upon glycosylation, the apparent molecular weight on western blots will increase to ~ 120 kDa.

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Antigen AC133, PROM1, Prominin-1, Prominin-like protein 1, PROML1