Anti-CD133 Antibody (A87952)

Name: Anti-CD133 Antibody
Brand: Antibodies.com
SKU: A87952
Price: 485.0 USD
Availability: InStock

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$485

Rabbit polyclonal antibody to CD133 for WB, ICC/IF and IP.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 5-8 business days.

Tags:

Knockout Validated Antibody

Specifications

Name

Anti-CD133 Antibody

Description

Rabbit polyclonal antibody to CD133.

Applications

WB, ICC/IF, IP

Dilutions

WB: 1:500-1:1,000, ICC/IF: 1:50-1:200, IP: 1:500-1:1,000

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 179-433 of human CD133 (NP_006008.1).

Sequence

ANHQVRTRIKRSRKLADSNFKDLRTLLNETPEQIKYILAQYNTTKDKAFTDLNSINSVLGGGILDRLRPNIIPVLDEIKSMATAIKETKEALENMNSTLKSLHQQSTQLSSSLTSVKTSLRSSLNDPLCLVHPSSETCNSIRLSLSQLNSNPELRQLPPVDAELDNVNNVLRTDLDGLVQQGYQSLNDIPDRVQRQTTTVVAGIKRVLNSIGSDIDNVTQRLPIQDILSAFSVYVNNTESYIHRNLPTLEEYDSY

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Molecular Weight

120 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.01% Thiomersal.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Synonyms

Antigen AC133, PROM1, Prominin-1, Prominin-like protein 1, PROML1

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (3)

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Western Blot - Anti-CD133 Antibody (A87952)

Western blot analysis of extracts of HCT116 cells, using Anti-CD133 Antibody (A87952) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-CD133 Antibody (A87952)

Western blot analysis of extracts from normal (control) and CD133 Rabbit polyclonal antibody knockout (KO) HCT116 cells, using Anti-CD133 Antibody (A87952) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

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Western Blot - Anti-CD133 Antibody (A87952)

Immunoprecipitation analysis of 200µg extracts of HT-29 cells using 3µg of Anti-CD133 Antibody (A87952). This Western blot was performed on the immunoprecipitate using Anti-CD133 Antibody (A87952) at a dilution of 1:1000.

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