Anti-CD13 Antibody [WM15] (A281974)

Anti-CD13 Antibody [WM15]

Mouse monoclonal [WM15] antibody to CD13.

This antibody recognises human CD13 also known as aminopeptidase N. CD13 is a single pass type II glycosylated integral membrane protein with a predicted molecular mass of ~110 kDa and an apparent molecular mass of ~150 kDa expressed by granulocytes, monocytes, fibroblasts, endothelial cells and by myeloid leukaemia cells. CD13 acts as a major cell surface receptor for group 1 coronoviruses which bind to a critical sequence encompassing amino acid residies 288-295. CD13 functions as an aminopeptidase enzyme, a metalloprotease present as both a membrane bound form and also a soluble aminopeptidase N. Mouse anti Human CD13, clone WM15 inhibits infection of cells by human coronavirus but not hepatitis C virus and inhibits aminopeptidase N activity of the CD13 molecule.

IHC-Fr, ELISA, Flow Cytometry, IP

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Human, Rhesus Monkey

Human AML cells.

Mouse

Monoclonal

WM15

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Human CD13 antibody, clone WM15 recognizes human CD13 also known as aminopeptidase N. CD13 is a single pass type II glycosylated integral membrane protein with a predicted molecular mass of ~110 kDa and an apparent molecular mass of ~150 kDa expressed by granulocytes, monocytes, fibroblasts, endothelial cells and by myeloid leukaemia cells (Bradstock et al. 1985). CD13 acts as a major cell surface receptor for group 1 coronoviruses (Breslin et al. 2003) which bind to a critical sequence encompassing amino acid residies 288-295 (Kolb et al. 1997).CD13 functions as an aminopeptidase enzyme, a metalloprotease present as both a membrane bound form and also a soluble aminopeptidase N.Mouse anti Human CD13, clone WM15 inhibits infection of cells by human coronavirus (Lachance et al. 1998) but not hepatitis C virus (Koutsoudakis et al. 2006) and inhibits aminopeptidase N activity of the CD13 molecule (Asmun et al. 1992).

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP-M, AP-N, APN, CD103, gp150, hAPN, HML-1 antigen, Integrin alpha-E, Integrin alpha-IEL, ITGAE, Microsomal aminopeptidase, Mucosal lymphocyte 1 antigen, Myeloid plasma membrane glycoprotein CD13, PEPN