Anti-CD13 Antibody [ER-BMDM1] (A280354)

Anti-CD13 Antibody [ER-BMDM1]

Rat monoclonal [ER-BMDM1] antibody to CD13.

This antibody recognises the murine homologue of human CD13, a ~160 kDa cell surface antigen with aminopeptidase N activity.In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13.Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Mouse

BALB/c bone marrow derived macrophages.

Rat

Monoclonal

ER-BMDM1

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD13 antibody, clone ER-BMDM1 recognizes the murine homologue of human CD13, a ~160 kDa cell surface antigen with aminopeptidase N activity.In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13.Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes.

Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, ANPEP, AP-M, AP-N, APN, CD103, gp150, hAPN, HML-1 antigen, Integrin alpha-E, Integrin alpha-IEL, ITGAE, Microsomal aminopeptidase, Mucosal lymphocyte 1 antigen, Myeloid plasma membrane glycoprotein CD13, PEPN