Hamster monoclonal [N418] antibody to CD11c (FITC).
Specificity
This antibody recognises the murine homolog of human CD11c, also known as Integrin Alpha X, a 150/90 kDa member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells.Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/BUF041B).
Reactivity
Mouse
Immunogen
Mouse spleen dendritic cells.
Host
Hamster
Clonality
Monoclonal
Clone ID
N418
Isotype
IgG
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Hamster anti Mouse CD11c antibody, clone N418 recognizes the murine homolog of human CD11c, also known as Integrin Alpha X, a 150/90 kDa member of the beta 2 integrin family. In mice, CD11c is primarily expressed by dendritic cells.Hamster anti Mouse CD11c antibody, clone N418 has been reported to enhance antigen specific responses when used to target dendritic cells in vivo (Wang et al. 2000).
Synonyms
CD11 antigen-like family member C, Integrin alpha-X, ITGAX, Leu M5, Leukocyte adhesion glycoprotein p150,95 alpha chain, Leukocyte adhesion receptor p150,95
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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