Anti-CD11b Antibody [OX-42] - BSA and Azide free (A281461)

Anti-CD11b Antibody [OX-42] - BSA and Azide free

Mouse monoclonal [OX-42] antibody to CD11b.

This antibody recognises rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglia in the brain. Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes as well as inhibit myelin binding and uptake.

IHC-Fr, Flow Cytometry, IP, IF

Flow Cytometry: 1:50 - 1:100, IHC-Fr: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

Resident rat peritoneal macrophages.

Mouse

Monoclonal

OX-42

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Rat CD11b, clone OX-42 recognizes rat CD11b, also known as integrin alpha-M, the receptor for the iC3b component of complement. CD11b is a 1151 amino acid single pass type 1 transmembrane glycoprotein posessing a single vWFA domain and multiple FG-GAP repeats. CD11b is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglia in the brain (Robinson et al.1986).Mouse anti Rat CD11b, clone OX-42 is reported to inhibit complement mediated rosettes (Robinson et al.1986) as well as inhibit myelin binding and uptake (van der Laan et al.1996).

CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, CR3A, Integrin alpha-M, ITGAM, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor