Anti-CD11b Antibody [MEM-174] (A85944)

Anti-CD11b Antibody [MEM-174]

Mouse monoclonal [MEM-174] antibody to CD11b.

The antibody recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.

Flow Cytometry, IP

Flow Cytometry: 1 µg/ml; Positive Control: peripheral blood lymphocytes, IP: Positive Control: granulocytes, CD11b/CD18 transfectants, Immunoprecipitation with the antibody MEM-174 was successfuly performed using immuno-affinity sorbents (minicolumns). The final immunosorbent should contain 1-5 mg of antibody/1 ml of sorbent gel (e.g. agarose beads). It is recommended to stimulate Positive Control material by LPS 1-2 days before to achieve better expression of target antigen.

Human

Human granulocytes.

Mouse

Monoclonal

MEM-174

IgG2a

Protein A chromatography.

1 mg/ml

165-170 kDa

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, CR3A, Integrin alpha-M, ITGAM, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor