Mouse monoclonal [ICRF44] antibody to CD11b (PE-Cyanine 7).
Specificity
This antibody recognizes an extracellular epitope of CD11b, a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes, and NK cells.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-Human Primates
Immunogen
Rheumatoid synovial cells and human monocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
ICRF44
Isotype
IgG1
Conjugate
PE-Cyanine 7
Excitation: 565nm, Emission: 778nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 7 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
165-170 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD11b Antibody [ICRF44] (PE-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
Separation of human neutrophil granulocytes (red-filled) from CD11b negative lymphocytes (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD11b Antibody [ICRF44] (PE-Cyanine 7) (4µl reagent per 100µl of peripheral whole blood).
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