Anti-CD11b Antibody [ED8] (PE) (A280337)

Anti-CD11b Antibody [ED8] (PE)

Mouse monoclonal [ED8] antibody to CD11b (PE).

This antibody recognises a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. It also recognises small ramified microglia in the central nervous system. No other cell types are positive for ED8. The recognized antigen is a heterodimer consisting of ~160 and ~95 kDa, belonging to the family of adhesion molecules CD11b/CD18, also designated as Mac-1 antigen or CR3. Mouse anti Rat CD11b antibody, clones ED7 (MCA618R) and ED8 may recognize closely related epitopes on the same molecule. Both clones ED7 and ED8 induce homotypic aggregation of granulocytes (Draskovic-Pavlovic et al. 1999).

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

Rat spleen cell homogenate with Freund's complete adjuvant.

Mouse

Monoclonal

ED8

IgG1

Ion exchange chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Rat CD11b antibody, clone ED8 recognizes a membrane antigen on rat macrophages, monocytes, dendritic cells and granulocytes. It also recognizes small ramified microglia in the central nervous system. No other cell types are positive for ED8. The recognized antigen is a heterodimer consisting of ~160 and ~95 kDa, belonging to the family of adhesion molecules CD11b/CD18, also designated as Mac-1 antigen or CR3. Mouse anti Rat CD11b antibody, clones ED7 (MCA618R) and ED8 may recognize closely related epitopes on the same molecule. Both clones ED7 and ED8 induce homotypic aggregation of granulocytes (Draskovic-Pavlovic et al. 1999).

CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, CR3A, Integrin alpha-M, ITGAM, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

This product is for research use only. It is not intended for diagnostic or therapeutic use.