Western Blot - Anti-CD11b Antibody [ARC0414] (A305854)
Western blot analysis of extracts of U-937 cells, using Anti-CD11b Antibody [ARC0414] (A305854) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 3min.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-CD11b Antibody [ARC0414] (A305854) at a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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