Anti-CD11b Antibody (A13522)

Name: Anti-CD11b Antibody
Brand: Antibodies.com
SKU: A13522
Price: 355.0 USD
Availability: InStock

Enlarge Image

$355

Rabbit polyclonal antibody to CD11b for WB, IHC and ICC/IF.

100% Guarantee

Price Match Guarantee

Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 7-10 business days.

Tags:

Microglia Marker

Specifications

Name

Anti-CD11b Antibody

Description

Rabbit polyclonal antibody to CD11b.

Applications

WB, IHC, ICC/IF

Dilutions

WB: 1:100-1:500, IHC: 1:50-1:200, ICC/IF: 1:50-1:200

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 145-340 of CD11b/ITGAM (NP_000623.2).

Sequence

PQEDSDIAFLIDGSGSIIPHDFRRMKEFVSTVMEQLKKSKTLFSLMQYSEEFRIHFTFKEFQNNPNPRSLVKPITQLLGRTHTATGIRKVVRELFNITNGARKNAFKILVVITDGEKFGDPLGYEDVIPEADREGVIRYVIGVGDAFRSEKSRQELNTIASKPPRDHVFQVNNFEALKTIQNQLREKIFAIEGTQT

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Affinity purification.

Concentration

Lot Specific

Molecular Weight

170 kDa

Product Form

Liquid

Formulation

Supplied in Phosphate Buffered Saline, pH 7.3, with 50% Glycerol and 0.05% Proclin 300.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

CD11 antigen-like family member B, Cell surface glycoprotein MAC-1 subunit alpha, CR-3 alpha chain, CR3A, Integrin alpha-M, ITGAM, Leukocyte adhesion receptor MO1, Neutrophil adherence receptor

Isotype Controls

Suitable Secondaries

See all Anti-Rabbit IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (5)

Enlarge Image

Western Blot - Anti-CD11b Antibody (A13522)

Western blot analysis of extracts of various cell lines, using Anti-CD11b Antibody (A13522) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.

Enlarge Image

Immunohistochemistry - Anti-CD11b Antibody (A13522)

Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD11b Antibody (A13522) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Immunohistochemistry - Anti-CD11b Antibody (A13522)

Immunohistochemistry analysis of paraffin-embedded mouse spleen using Anti-CD11b Antibody (A13522) at a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Enlarge Image

Immunofluorescence - Anti-CD11b Antibody (A13522)

Immunofluorescence analysis of TF-1 cells using Anti-CD11b Antibody (A13522) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).